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. 2009 Apr 7;3(4):e407. doi: 10.1371/journal.pntd.0000407

Figure 6. Memory phenotypes of IFN-γ producing cells from recovered melioidosis cases vs. seropositive healthy control subjects in response to B. pseudomallei in vitro.

Figure 6

Whole blood samples were incubated with whole B. pseudomallei for 12 hours and stained for intracellular IFN- γ vs. three immune cell surface markers (Tri-color anti-CD4 or CD8, FITC-anti-CCR7 and PE-anti-CD45RA), gated on IFN-γ+ cells. The distribution of memory phenotype subsets i.e., central memory (TCM, CD45RA−CCR7+), effector memory (TEM, CD45RA-CCR7−) and terminally differentiated T effector memory (TEMRA, CD45RA+CCR7−) of (A) 16 recovered melioidosis (R) vs. 7 seropositive individuals (S+), (B) recovered melioidosis with the history of septicemic (SR, n = 12) vs. localized (LR, n = 4) melioidosis. Horizontal lines indicate mean±SE values of the group.