Figure 4.

The Larger PDCB Family of Pd Proteins.

(A) Phylogenetic tree of the X8 domains of Arabidopsis PDCB-related proteins. The analysis, which includes X8 domains from 30 PDCB structural homologs, is presented as a midpoint rooted phylogenetic tree of proteins containing X8 as their only identifiable functional domain; 1,3-β-glucanases are excluded from this analysis. X8 domains from proteins with (bold) and without GPI anchors are listed as Arabidopsis Genome Initiative numbers. Olive OLE-e10 is included and shows closer similarity to the clade of non-GPI X8 proteins than to the GPI-anchored proteins. Only bootstrap values >70% are shown. The PDCB subfamily is indicated. Proteins tested for their subcellular targeting as YFP fusion proteins are underlined. The sequences used to generate this phylogeny are presented in Supplemental Data Set 1 online.

(B) Transient expression of 35SproYFP-PDCB2 in N. benthamiana leaves showing fluorescence as punctate spots on the walls of epidermal pavement cells.

(C) Transgenic expression of 35SproYFP-PDCB3 in Arabidopsis showing fluorescence as punctate spots on the walls of epidermal pavement cells.

(D) Transgenic expression of 35SproYFP-At1g69295 in Arabidopsis showing fluorescence as punctate spots on the walls of epidermal pavement cells.

(E) Transgenic expression of 35SproYFP-At3g58100 in Arabidopsis showing fluorescence as punctate spots on the walls of epidermal pavement cells. Bars in (B) to (E) = 10 μm.