Ag+ Reduces Low K+-Induced ROS Production and Low K+-Induced Root Hair Elongation in ein2-1.
(A) ROS production increases in ein2-1 under low potassium conditions, and this increase is blocked by Ag+. Pseudocolor images (pixel intensity) of ROS fluorescence are shown for K+-sufficient (+K, 1.75 mM K+) roots and for K+-starved (−K, no K+) roots with or without Ag+ (1, 50 μM; 2-h treatment). Three-day-old ein2-1 roots were used 24 h after K+ deprivation. Bar = 200 μm; bar in inset = 100 μm.
(B) Quantified data of images shown in (A) (n = 10 to 15 images of roots ± se).
(C) Root hair length of ein2-1 plants in the absence (−) or presence (+) of 150 nM Ag+ under K+-sufficient (+K, 5 mM K+) and K+-deficient (−K, no K+) conditions. Root hair length of 3-d-old ein2-1 roots was measured 22 h after K+ deprivation and Ag+ treatment (n = 10 seedlings, means ± se). Means that have different letters at the top are significantly different (P < 0.05; t test).