Figure 3.

Role of phyA in the Phosphorylation of GFP-FHY1 and Endogenous FHY1.

(A) to (D) Immunoblot analysis of proteins extracted from seedlings grown for 3 d in darkness and then transferred to red light for the time periods indicated in minutes (m). Seedlings tested were as follows: 35S:GFP-FHY1 and 35S:GFP-FHY1/phyA-1 (A), wild type and phyA-1 (B), 35S:GFP-FHY1/phyB-1 and 35S:GFP-FHY1/cry1 (C), and phyB-1, phyBDE, and cry1 cry2 (D).

(E) Three-day-old dark-grown wild type, phyA-1, and cry1cry2 seedlings were transferred to blue light (B) for the time periods indicated.

In (A) to (E), immunoblots were probed with anti-FHY1 antibody. Asterisks indicate slower migrating phosphorylated forms of GFP-FHY1 and FHY1.