Figure 4.

Effect of phyA Mutations on FHY1 Phosphorylation.

Immunoblot analysis of proteins extracted from seedlings grown for 3 d in darkness and then transferred to red light for the time periods indicated in minutes (m). Seedlings tested were as follows: wild type (ecotype Columbia), phyA-300D, and phyA-302GFP/phyA-211 (A); wild type (ecotype RLD) and phyA-105 (B); and wild type (ecotype Ler), phyA-201, phyAOX, and phyA S598A (the last two in phyA-201 background) (C). Immunoblots were probed with anti-FHY1 antibody and analyzed for appearance of the slow migrating phosphorylated forms of FHY1 (indicated by asterisks).