Figure 2.

Analysis of Tl Allelic Variants.

(A) Gene structure represented as boxes for exons and lines for introns. The white box indicates the coding region for the homeodomain, and the gray box represents the leucine zipper region. The positions of the ATG start and TGA stop codons are shown. Vertical arrows indicate nucleotide changes present in various tl alleles.

(B) Tl gene expression in wild-type accessions and mutant alleles analyzed by PCR after reverse transcription of RNA extracted from 3-week-old shoot tips. Tl gene-specific primers flanking the second intron were predicted to amplify a 424-bp product from cDNA and a 645-bp product from genomic DNA present in the samples. Lane 1, wild-type Witham Wonder; lane 2, tl-pet JI 32; lane 3, wild-type JI 1194; lane 4, tl-w JI 1197; lane 5, wild-type JI 516; lane 6, tl-12 JI 1373; lane 7, wild-type JI 2224; lane 8, tl-13 JI 3128; lane 9, wild-type JI 3131; lane 10, tl-16 JI 3130; lane 11, wild-type JI 2282; lane 12, tl-10 FN 2086/3; lane 13, wild-type Torsdag 5839; lane 14, tl-17 MC1a/1. Primers specific for the garden pea Argonaute gene (Ago) were used as controls for PCR amplification.