Figure 4.

Increased Tolerance of 35S:Rma1H1 Arabidopsis Transgenic Plants to Water Stress.

(A) RT-PCR analysis of 4-week-old wild-type and four independent 35S:Rma1H1 T4 transgenic plants (lines #7, #9, #18, and #22).

(B) Wild-type and transgenic lines were grown in pots for 3 weeks under normal growth conditions. Thereafter, water was withheld for 12 d, followed by rewatering for 3 d. Dehydration tolerance was assayed as the capability of plants to resume growth when returned to normal conditions following water stress. The survival rate of wild-type and four independent transgenic lines are shown. Error bars are ±sd (n = 5).

(C) Water loss of wild-type and 35S:Rma1H1 leaves before and after drought stress. Water loss is expressed as the percentage of initial fresh weight of detached leaves. Error bars are ±sd (n = 10).