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. 2009 Feb;21(2):479–493. doi: 10.1105/tpc.108.059550

Figure 6.

Figure 6.

Highly Purified ANN33/35 Preparation Forms a K+-Permeable Conductance in Planar Lipid Bilayers.

(A) Representative current traces recorded in response to the voltage protocol (as in Figure 5B) applied from a 0 mV baseline. A POPE:cholesterol:POPS (5:3:2) bilayer was used. ANN33/35 preparation (3 μg) was added to the cis-chamber comprising 200 mM KCl, pH 6. The trans-chamber comprised 50 mM KCl, pH 6.0, ± 50 mM TEA+. Top panel, prior to annexin addition. Middle panel, current generated by addition of ANN33/35. Bottom panel, effect of 50 mM TEA+ in the trans-chamber.

(B) Mean ± se I-V relationships for ANN33/35 preparation (closed circles; n = 3) and effect of 50 mM TEA+ (open circles; n = 3). EK was of −31 mV, and ECl was +31 mV.