Figure 4.

Conidiation of the Δlhs1 Mutant and MHP1 Expression.

(A) Conidiation was compared among the wild type, the Δlhs1 mutant, and cp19 (an ectopic transformant) after growth on oatmeal agar medium (OMA) for 13 d. The asterisk denotes 0.4 ± 0.1. The values are the mean ± sd of three replicates.

(B) Transcription of MHP1 during conidiation in the Δlhs1 mutant was compared with that in the wild-type strain by quantitative RT-PCR. The profiles were normalized using CYP1 (a gene encoding cyclophilin as endogenous control) transcripts and calibrated against the vegetative growth profile. Both strains were sampled at day 10 (conidiating mycelia on OMA) and at day 3 (mycelia in complete liquid medium). The quantitative RT-PCR reactions were repeated twice independently with three replicates, and a representative set of data is presented. The error bars are sd of three replicates.

(C) Conidia development was observed under a light microscope 16 (top) and 24 h (bottom) after induction of conidiation under cover slips. Schematic drawings of conidiophores and conidia from each culture at 24 HAI were prepared based on microscopy observations. Bar = 100 μm.