Figure 7.

Expression of Salt-Regulated Genes in Srlk Mutants.

Effect of Srlk mutation on the expression of early induced salt-responsive genes was determined in roots treated with 150 mM NaCl during 6 h. RNA samples from srlk1-1 and srlk1-2 homozygous lines and their corresponding segregating heterozygous siblings were analyzed by real-time RT-PCR. Histograms represent the ratio of expression levels of each gene in homozygous mutant lines in relation to their heterozygous sibling (black columns). The genes studied were Zpt2-1, CDPK3, and Srlk in control (A) and salt stress conditions (B). Values were normalized against the actin gene.