TABLE 1.

Results of immunohistochemical analysis of sections of hearts

Treatment group	Animals	Total sections	Percentage positive cells of total cells (n)
			TUNEL	Cleaved caspase 3	ssDNA
1 h ischemia, 24 h reperfusion	3	9	38.6% (n=304)†	0 (n=100)	1.0% (n=97)
4 h ischemia, 24 h reperfusion	3	9	48.5% (n=362)†	0 (n=100)	0.7% (n=91)
24 h ischemia	3	9	67.2% (n=294)*,†	0 (n=100)	2.8% (n=80)

^*P = 0.009 compared with results in the 4 h of ischemia and 24-h reperfusion group and 0.001 compared with those in the 1 h of ischemia and 24-h reperfusion group. 

^†P < 0.001 compared with results for cleaved caspase 3 and for ssDNA.