Figure 3.
CN induces TRPA1-like currents in mouse DRG neurons. A) TRPA1-like current-voltage curves of a representative mouse DRG neuron before activation (black trace), activation by 100 μM CN (green trace), and inhibition by ruthenium red (10 μM, red trace) in whole-cell configuration. Vholding = 0 mV to minimize voltage-gated channels. Currents were measured with a voltage ramp from −100 to +100 mV over 100 ms at 0.5-Hz intervals. Intracellular Cs-based solution with 10 mM EGTA was used. B) Average native TRPA1-like currents at −80 and +80 mV in mouse DRG neurons superfused with 100 μM CN (black bar), followed by ruthenium red (RuRed; 10 μM) as described for Fig. 1B (n=4 of 16 neurons). Baseline current was subtracted for each trace. C) hTRPA1 current-voltage curves before activation (black trace), at maximal activation by 10 μM CN (green trace), and after inactivation phase (blue trace) in whole-cell configuration. Currents were measured with a voltage ramp from −80 to +80 mV over 100 ms at 0.5 Hz intervals, Vholding= 0 mV. Intracellular Cs-based solution with 10 mM EGTA was used. D) Averaged TRPA1 currents at −80 and +80 mV in hTRPA1-transfected HEK-293T cells superfused with 10 μM CN (black bar) as described for Fig. 1C (n=4).