Fig. 2.

Design of multipromoter based screen for GR modulators. (A) Cells were transfected with the indicated fluorescent protein reporter plasmids and plated into 96-well dishes the next day. (B) Two hours later, dex (100 nM) and library compounds (2.5 μM) were added to the cells via liquid-handling robot. (C) The next day, fluorescence for each reporter was measured with a plate reader. (D) Hits were selected based on predefined criteria (see Materials and Methods) and retested in the screening assay. (E) Fresh compounds of repeat hits were obtained and retested in FP assays and dose–response studies. (F) Selected compounds were analyzed in further detail.