Fig. 6.

Impact of selected GR modulators on GR-mediated cytokine repression and adipogenesis. (A) Relative mRNA levels of the indicated target genes were determined by using qPCR. Cells were treated for 4 h (Cin B) or 24 h (Para and Hp). Doses used were TNF-α (2.5 ng/mL); Cin B (50 nM), Para (10 μM), and Hpg (2.5 μM). *, P ≤ 0.05. (B–F) Oil red staining of 3T3-L1 adipocytes. Cells were grown to confluence and then treated with 250 nM dex, 0.5 μM 3- isobutyl-1-methylzanthine, and .83 μM insulin for 48 h, followed by 5–7 days of additional culture as described (38). Various compounds as indicated were added to the culture media concomitant with the 48 h of dex treatment. (G and H) C2C12 cells were placed in differentiation medium (G) and differentiation medium plus 50 nm Cin B (H) for 72 h. Myosin heavy chain expression was visualized with indirect immunofluorescence.