Phytochrome inactivation by FR radiation reduces the sensitivity to MeJA elicitation of selected components of the jasmonate response. (A–C) FR reduces the response of HEL, PDF1.2, and their regulator, ERF1. (D) Neither FR nor MeJA affect the expression of COI1. (E and F) The responses of MYC2 and its target VSP1 to MeJA stimulation are not affected by FR. (G) FR up-regulates JAZ10, one of the transcriptional regulators that repress jasmonate responses (for data on other JAZ genes, see Fig. S6). Amb, ambient light; FR, FR added to ambient light to simulate the effect of neighbor proximity in a canopy of low-leaf area index. The inset table shows a summary of the statistical significance of the main effects of FR and MeJA, and the FR × MeJA interaction. Red lettering highlights relevant statistical effects. Expression data are normalized to the expression level detected in the Ambient × Control combination. Thin bars indicate ± SEM (n = 4 replicates, see Materials and Methods). The same patterns of response were observed in samples taken 24 h after elicitation (data not shown).