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. 2009 Mar 5;106(12):4822–4827. doi: 10.1073/pnas.0806647106

Fig. 3.

Fig. 3.

Bystander effect and migration of MSCs. (A–F) Human Gli36-EGFRvIII glioma cells were labeled with DI1 and MSCs were labeled with calcein-AM, mixed, and FACS-sorted after 5 min and after 3 h. FACS-sorted plots and graphs reveal different populations of labeled cells after 5 min (A, C, and D) and 3 h (B, E, and F). (G and H) Photomicrographs of MSCs expressing tdTomato (G) and human Gli36-EGFRvIII glioma cells expressing GFP-Fluc (H). MSCs expressing tdTomato were implanted intracranially at a 1-mm distance from established human gliomas expressing GFP-Fluc. (I and J) Photomicrographs showing MSC-tdTomato (red) and gliomas (green) on day 2 (I) and day 10 (J) in brain sections and by intravital microscopy on day 10 after MSC implantation (K). (L–O) Immunohistochemistry on day-14 brain sections from Gli36-EGFRvIII-glioma bearing mice implanted with MSCs expressing tdTomato. Representative images of brain sections immunostained for Ki67 (L), nestin (M), GFAP (N), and MAP-2 (O). (Green, GFP expression; blue, nestin, Ki67, GFAP, or MAP-2 expression; original magnification: I and J, ×10; K–O, ×20.)