Fig. 4.

c-Abl KO B cells have WT levels of NF-κB subunits and DNA-binding activity. (A) Western blot analysis of splenic B cell lysates. Membranes were probed with antibodies against c-Rel, p65 and actin. (B) NF-κB DNA-binding activity was assayed by EMSA. B cells from WT (+/+) or mutant (−/−) spleens were fractionated and increasing amounts of nuclear lysates were incubated with ³³P-labeled NF-κB-specific probe (probe), unlabeled probe + labeled probe (comp) or unlabeled scrambled probe + labeled probe (neg).