Figure 6.

Enhanced expression of cytokine receptors for TNF-α and IL-1β in RVM neurons at 14 d after CCI-ION. A, C, Immunostaining shows distribution of TNFR1 (A; red) and IL-1RI expression (B; red) in low power of a tissue section at the rostral medulla level (a) and enlarged RVM region (b) corresponding to the small rectangle area in a. The arrowheads in b and c indicate single-labeled neurons. Double labeling shows TNFR1-labeled (Ab) and IL-1RI-labeled cells (Cb) are immunoreactive to NeuN (Ac, Cc; green), suggesting their expression in the RVM neurons (Ad, Cd; arrows). B, D, Western blots illustrating that the levels of TNFR1 (B) and IL-1RI (D) are increased in the RVM tissues at 14 d in CCI-ION rats compared with sham-operated rats (p < 0.05; n = 3 per group). *p < 0.05 versus naive in B and D. Scale bars: Aa, Ca, 0.1 mm; Ab–d, Cb–d, 0.03 mm. Error bars indicate SEM.