Fig. 1.
PCDH-PC mRNA expression in human prostatic tissue, hormone sensitive, and apoptosis resistant cell lines, a: Expression of PCDH- PC mRNA was investigated by semi-quantitative RT-PCR and by comparison with an internal control, the TBP mRNA. PCDH-PC mRNA expression was higher in apoptosis resistant lines LNCaP-TR and LNCaP-SSR compared to LNCaP parental cell line, b: Relative expression of PCDH-PC mRNA in prostatic tissues was also determined by semi-quantitative RT-PCR. Values corresponded to the mean of PCDH-PC expression levels in RNAs extract from different groups: the peripheral (n = 7), central (n = 9), and transitional (n = 6) zones of the normal prostate, the benign hyperplastic prostate (n = 15), untreated (n = 10), and treated (n = 8) prostate tumors and hormonal refractory patients (n = 9). Of note, PCDH-PC expression is significantly upregulated in hormone failure group as compared to either non-CaP, treated, or untreated CaP groups.