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A sample of technical variables shown to play a role in mRNA expression studies
| Time between sample removal and RNA |
| isolation |
| RNA extraction efficiency |
| Amplification yield |
| Labeling yield |
| Hybridization efficiency |
| Dye bias |
| Fluorescence gain bias |
| cDNA array printing pins |
| Properties of probe plates |
| Inter-individual differences |
| Non-linear cross-talk effects |
| Pooling/non-pooling bias |
| Genome annotation irregularities |
| Using correct analytical tools |
