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. 2009 Feb 12;9:3. doi: 10.1186/1472-6769-9-3

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Copyright © 2009 Bertinetti et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Improvements on cAMP analogs for the purification of all R-subunit isoforms. Comparison of the protein yield eluted from the agarose with cGMP (red bars) followed by cAMP (blue bars) indicated on the plot. Data were normalised to the total protein yield from both elution steps using Sp-8-AEA-cAMPS agarose as determined by BCA assay. The lower panel shows a SDS-PAGE of samples from each elution fraction (equal volumes of original fractions, cGMP elution red framed, cAMP elution blue framed). M: molecular weight marker (Page-Ruler Unstained Protein Ladder, Fermentas).