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. 2009 Feb 12;9:3. doi: 10.1186/1472-6769-9-3

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Copyright © 2009 Bertinetti et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Affinity enrichment of components of the cAMP-pathway using agonist and antagonist agaroses. Proteins from pig brain tissue were enriched using agonist (Sp-8-AEA-cAMPS) and antagonist (Rp-8-AHDAA-cAMPS) agaroses and subsequently eluted with cAMP. Fractions were separated on a 12% SDS-PAGE. supernatant: soluble protein fraction of pig brain after lysis, filtration and centrifugation; agonist/antagonist: protein eluted from the agonist/antagonist agarose with 25 mM cAMP. C-subunits (42 kDa), R-subunits RI (50 kDa) and RII (52 kDa) subunits are indicated, each identified by MS (data not shown). M: molecular weight marker (Page-Ruler, unstained protein ladder, Fermentas).