Table 1.

Three nucleotide motif mutability index (MI)^a for WT and mutant AID (S38A and S43P) at 2.5 min incubation with lacZα gapped region.

	MI
motif	WT	S38A	S43P	pb
hot spots
AAC	2.5	1.5	1.6	0.41
AGC	1.3	2.1	2.0	0.06
TAC	3.4	2.0	2.2	0.22
TGC	2.6	2.2	2.6	0.99
MI average (WRC)	2.5	1.9	2.1
cold spots
CCC	0.28	0.52	0.44	0.65
CTC	0.45	0.76	0.53	0.99
GCC	0.05	0.18	0.05	0.07
GTC	0.32	0.67	0.00	0.99
MI average (SYC)	0.28	0.53	0.26
intermediates
ACC	1.2	0.32	0.35	0.02
ATC	1.0	0.63	0.71	0.97
CAC	1.4	0.91	0.96	0.31
CGC	0.5	1.3	1.4	0.004
GAC	0.24	0.5	0.35	0.79
GGC	0.23	0.78	1.0	0.0001
TCC	0.48	1.1	1.1	0.75
TTC	0.53	0.71	0.71	0.46
MI average	0.70	0.78	0.82

^aThe MI is defined as the number of times a given trinucleotide motif within a segment of DNA contains a mutation, divided by the number of times the oligonucleotide would be expected to be mutated for a mechanism with no sequence bias.

^bThe p-values were calculated by the paired Wilcoxon test (see Pham et al. 2008). Adapted from Pham et al. (2008).