Table 2.
Peak fluorescence values for vegetative B. subtilis cells stained with DiOC6(3)*
| Cell treatment | Peak fluorescence1 |
|---|---|
| None | 4 |
| + DiOC6(3) | 155 |
| + DiOC6(3) and FCCP | 8 |
| heat2 — then DiOC6(3) | 6 |
| + DiOC6(3) then heat2 | 21 |
*
Vegetative B. subtilis PS533 cells were prepared, stained and analyzed by flow cytometry as described in Methods.
1
Peak fluorescence values in arbitrary units were determined from the position of the major peak (generally > 85% of all cells) of fluorescent cells in flow cytometry. All values are averages of at least duplicate experiments and individual values differed by < 25% from the averages.
2
Cells were heated for 30 min at 80°C before or after DiOC6(3) addition.