Figure 5. ES, CP, and acute clones show no differential susceptibility to CCL5, TAK-779, or ENF.

(A–C) ES, acute, and chronic clones were used to infect Affinofile cells induced to express approximately 125,000 molecules CD4 and approximately 1,274 molecules CCR5/cell. (A) CCL5 was added in concentrations ranging from 0.1–50 nM, incubated for 1 h, and infected. Plots represent percent replication relative to no drug. 50 percent inhibitory concentrations of drug (IC₅₀ values) were computed from the curves and plotted for acute, chronic, ES, and Yu-2 or SF162 envelopes. (B) TAK-779 was added to the induced Affinofile cells at concentrations ranging from 0.1 nM–1 µM. Plots were used to determine IC₅₀ values for each envelope. (C) Enfuvirtide was added to cells at concentrations ranging from 0.1 nM–1 µM.