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Changes in the amplitude of backbone motions (ΔS2) for the CI2 hydrophobic core mutants based on Lipari-Szabo model free analysis of 15N relaxation data acquired at 25 °C. Plots in the left column are for folding nucleus mutants, whereas the right column shows data for core mutants that are not part of the canonical folding nucleus. In all plots, the site of mutation is indicated by a red bar, and residues exhibiting significant changes in dynamics are colored cyan.
