Figure 2.

Increase of direct yellow fever virus (YFV) inactivation by the presence of essential oil in the supernatant of virus-infected cells culture. YFV previously incubated with serial dilutions of essential oil (Figure 1) was replicated in Vero cells at 37°C in M-199 medium containing varied concentration of essential oil. Virus titers in supernatants of cell cultures were determined by plaque assays method at 48 h after adsorption. The data represent the means for three replicate samples of two independent experiment. Error bars indicate standard deviations.