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Character of the UL31 RNA expression in infected DEF. Total RNA was extracted from the cells for determination of UL31 mRNA expression by RT-PCR assays as described in the Methods. The upper panel shows RNA from uninfected DEF (UN) and infected DEF at different times p.i. (6, 12, 24, 36, 48, 60 and 72 h), amplified by RT-PCR. Marker: molecular mass marker DL2000 (TaKaRa). The lower panel shows β-actin, which was run as an RNA-competence control.
