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Extraocular muscle (EOM) contains 15 times more side population (SP) cells than tibialis anterior (TA). Total mononuclear cells from EOM and TA were labeled with Hoechst 33342 in the presence and absence of verapamil. SP cell population was selected with a third log cutoff between main population and SP in the density plots (see Supplemental Fig. S1). Values are mean ± SD numbers of SP cells per gram of tissue in EOM compared with TA; n = 7. *P < 0.05.
