Fig. 1.

Continued ingestion an survival rates on NaCl-supplemented diet. A: example of Indigo carmine in gut after 3 h of feeding with 4% NaCl food (left) compared with 6 h gut that would be scored as clear (right). B: ingestion of high-salt food over time. Flies in 0, 4, and 5% NaCl food, which contained the blue dye Indigo Carmine, were dissected after 1, 3, and 6 h and scored for blue gut contents. C: survival rates in days for flies fed with 0, 3, 4, 5, 6, 7.5, and 9% NaCl compared with starvation. Increasing NaCl significantly reduces survival [Kaplan-Meier analysis with log-rank test for trend, taking P = 0.05 as the critical level (2-tailed)]. *Significantly different from the starvation control.