Fig. 7.

Effect of modulation of tubule CG2196 expression on whole fly survival after salt challenge. Flies were allowed to lay eggs on 4% NaCl diet, then the numbers of progeny reaching adulthood were counted and compared with survival of RNAi, eYFP, or c42 parental lines. The c42 line drives expression selectively in tubule principal cells. For each experimental condition, 2 independent replicates are shown. A and B: tubule-specific RNAi against CG2196 enhances survival on 4% salt. C and D: tubule-specific overexpression of eYFP-tagged CG2196 has no significant impact on expression relative to parental controls. *Significant change from control (Student's t-test, taking P < 0.05 as significant).