Table 1.
Iterative Group Analysis of gene families up- and downregulated by dietary salt stress
| Gene Ontology Code | Group |
% Gene Family Changed at Sample Time, h |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 4 | 8 | 16 | 32 | |||||||
| A. Upregulated gene families | ||||||||||
| 0008745-N-acetylmuramoyl-l-alanine amidase activity | immune | 25 | ||||||||
| 0006955-immune response | immune | 11 | ||||||||
| 0045087-innate immune response | immune | 14 | ||||||||
| 0042742-defense response to bacterium | immune | 14 | ||||||||
| 0050829-defense response to Gram-negative bacterium | immune | 18 | ||||||||
| 0050830-defense response to Gram-positive bacterium | immune | 9 | ||||||||
| 0019731-antibacterial humoral response | immune | 25 | ||||||||
| 0009253-peptidoglycan catabolic process | immune | 25 | ||||||||
| 0042834-peptidoglycan binding | immune | 25 | ||||||||
| 0030342-1-a,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity | 100 | |||||||||
| 0004558-alpha-glucosidase activity | sugar | 25 | 42 | 42 | 50 | |||||
| 0043169-cation binding | 10 | 10 | 16 | 14 | ||||||
| 0004613-phosphoenolpyruvate carboxykinase (GTP) activity | 100 | 100 | 100 | 100 | ||||||
| 0004611-phosphoenolpyruvate carboxykinase activity | 100 | 100 | 100 | 100 | ||||||
| 0006094-gluconeogenesis | sugar | 40 | ||||||||
| 0017076-purine nucleotide binding | 40 | |||||||||
| 0004364-glutathione transferase activity | 13 | |||||||||
| 0008643-carbohydrate transport | sugar | 9 | 9 | |||||||
| 0005355-glucose transmembrane transporter activity | sugar | 18 | 24 | |||||||
| 0005351-sugar:hydrogen ion symporter activity | 11 | 14 | ||||||||
| 0006006-glucose metabolic process | sugar | 27 | 27 | 27 | ||||||
| 0005792-microsome | 4 | |||||||||
| 0009063-amino acid catabolic process | AA | 17 | ||||||||
| 0016798-hydrolase activity, acting on glycosyl bonds | sugar | 11 | ||||||||
| 0005996-monosaccharide metabolic process | sugar | 22 | 17 | |||||||
| 0009113-purine base biosynthetic process | 75 | 75 | ||||||||
| 0006144-purine base metabolic process | 9 | 15 | ||||||||
| 0003939-L-iditol 2-dehydrogenase activity | 100 | 100 | ||||||||
| 0006164-purine nucleotide biosynthetic process | 40 | 50 | ||||||||
| 0006189-'de novo' IMP biosynthetic process | 43 | 57 | ||||||||
| 0006012-galactose metabolic process | sugar | 50 | 50 | |||||||
| 0008652-amino acid biosynthetic process | AA | 10 | ||||||||
| 0004477-methenyltetrahydrofolate cyclohydrolase activity | 100 | |||||||||
| 0009072-aromatic amino acid family metabolic process | AA | 38 | ||||||||
| 0004488-methylenetetrahydrofolate dehydrogenase. (NADP+) activity | 100 | |||||||||
| 0006559-L-phenylalanine catabolic process | AA | 50 | ||||||||
| B. Downregulated gene families | ||||||||||
| 0005730-nucleolus | 12 | |||||||||
| 0008143-poly(A) binding | 20 | |||||||||
| 0005616-larval serum protein complex | 33 | |||||||||
| 0005344-oxygen transporter activity | 14 | |||||||||
| 0045735-nutrient reservoir activity | 25 | |||||||||
| 0046870-cadmium ion binding | 67 | 67 | ||||||||
| 0005786-signal recognition particle, endoplasmic reticulum targeting | 27 | |||||||||
| 0004035-alkaline phosphatase activity | 23 | |||||||||
| 0005316-high affinity inorganic phosphate:sodium symporter activity | 22 | |||||||||
| 0005048-signal sequence binding | 75 | |||||||||
| 0006621-protein retention in ER | 100 | |||||||||
| 0050830-defense response to Gram-positive bacterium | immune | 17 | ||||||||
| 0045087-innate immune response | immune | 15 | 10 | |||||||
| 0006955-immune response | immune | 13 | 7 | |||||||
| 0042742-defense response to bacterium | immune | 16 | 11 | |||||||
| 0050829-defense response to Gram-negative bacterium | immune | 27 | 18 | |||||||
| 0019731-antibacterial humoral response | immune | 32 | 21 | |||||||
| 0005784-translocon complex | 43 | 43 | ||||||||
| 0042562-hormone binding | 67 | 67 | ||||||||
| 0007321-sperm displacement | reprod. | 50 | 75 | |||||||
| 0045861-negative regulation of proteolysis | 50 | 63 | ||||||||
| 0007610-behavior | 13 | 23 | ||||||||
| 0004182-carboxypeptidase A activity | 17 | |||||||||
| 0007305-vitelline membrane formation in chorion-containing eggshell | reprod. | 57 | ||||||||
| 0007343-egg activation | reprod. | 33 | ||||||||
| 0008316-structural constituent of vitelline membrane | reprod. | 100 | ||||||||
| 0004181-metallocarboxypeptidase activity | 33 | |||||||||
| 0004263-chymotrypsin activity | 18 | 23 | ||||||||
| 0004806-triacylglycerol lipase activity | 11 | 16 | ||||||||
| 0008970-phospholipase A1 activity | 30 | 20 | ||||||||
| 0016045-detection of bacterium | immune | 33 | ||||||||
| 0004867-serine-type endopeptidase inhibitor activity | 12 | |||||||||
| 0016298-lipase activity | 16 | |||||||||
| 0046692-sperm competition | reprod. | 50 | ||||||||
| 0042600-chorion | reprod. | 38 | ||||||||
| 0005213-structural constituent of chorion | reprod. | 78 | ||||||||
| 0007306-eggshell chorion formation | reprod. | 36 | ||||||||
Gene families that are significantly (by iterative group analysis, Ref. 9) overrepresented in either the up- or downregulated groups are ordered by time of onset and are highlit with the following names: immune, immune/defence response; reprod, reproduction; sugar, carbohydrate metabolism; AA, amino acid metabolism. Note that, because of the limitations and hierarchical nature of gene ontology annotation, there is substantial overlap between the genes reported under related headings. Nonetheless, the trends should be clear.