Table 3.
Genes selected for further study
|
A. FlyAtlas expression levels across multiple tissues | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene | Brain | Head | Tag | Crop | Midgut | Tubule | Hindgut | Ovary | Testis | Acc | Car | L_fat | Fly |
| CG2196 | 7 | 4 | 5 | 7 | 8 | 7,248 | 70 | 4 | 6 | 11 | 21 | 5 | 275 |
| CG6484 | 6 | 22 | 4 | 7 | 2,738 | 12 | 32 | 4 | 8 | 523 | 161 | 6 | 178 |
| sugarbabe | 10 | 197 | 10 | 8 | 584 | 455 | 95 | 5 | 6 | 8 | 371 | 550 | 137 |
|
B. qPCR validation of CG2196 and CG6484 expression in Malpighian tubules, guts, and heads | |||
|---|---|---|---|
|
Relative Expression Level (w.r.t. rp49) |
|||
| Gene | Head | Midgut | Malpighian tubules |
| CG2196 | 4.8×10−5±1.2×10−5 | 3.6×10−5±4.5×10−6 | 0.129±0.008 |
| CG6484 | 2.38×10−7±1.8×10−8 | 8.7×10−5±1.5×10−5 | 3.3×10−8±4.2×10−9 |
In A, the FlyAtlas expression profile across multiple tissues is shown. The maximal signal for each gene is shown in bold. Details of the FlyAtlas data set have been published previously (11). In B, data are shown as means ± SE (n = 3 independent biological replicates).