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. 2009 Apr 3;4(4):e5012. doi: 10.1371/journal.pone.0005012

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Lucas et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic presentation of the CbfA protein. CbfA (1000 amino acids) contains the following domains: JmjC: jumonji domain; ZF: zinc fingers; NRD: asparagin-rich domain; AT: AT-hook; CTD: carboxy-terminal domain. GFP-CbfA^724–998 represents a protein expressed from plasmid pPT132-CTD. The amino acid sequence surrounding the AT-hook is indicated. The AT-hook itself is indicated by the red box. Amino acids contributing to nuclear localization are written in red color and bold. Mutations introduced into the GFP-CbfA^724–998 protein to test for nuclear localization and in vivo function of the AT-hook, respectively, are indicated below the arrows (see text for details). (B) Shown is the expression in JH.D cells of GFP-CbfA^724–998 wildtype protein and mutants GFP-CbfA^724–998(R812A), GFP-CbfA^724–998(K799N/R800G), GFP-CbfA^724–998(P801K), and GFP-CbfA^724–998(P801K/R812A). Cells were fixed and analyzed by confocal laser scanning microscopy. Scale bar is 10 µm.