Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed to detect Crimean-Congo hemorrhagic fever (CCHF) virus-specific immunoglobulin M (IgM) in human serum samples. For this test, a heat-inactivated antigen was prepared from the brains of suckling mice infected with CCHF virus. The IgM-capture ELISA proved more sensitive than indirect fluorescence tests for IgM to this virus. A human serum containing high-titer IgM to CCHF virus was used for an antigen-capture ELISA to detect this virus in heat-inactivated suspensions of virus-infected ticks. The antigen-capture ELISA appeared to be as sensitive as virus isolation in suckling mice. The studies described suggest that the IgM-capture ELISA and the antigen-detection ELISA should provide a rapid and sensitive diagnosis of human CCHF virus infection and should be useful in ecologic studies of this virus.
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Selected References
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- Burney M. I., Ghafoor A., Saleen M., Webb P. A., Casals J. Nosocomial outbreak of viral hemorrhagic fever caused by Crimean Hemorrhagic fever-Congo virus in Pakistan, January 1976. Am J Trop Med Hyg. 1980 Sep;29(5):941–947. doi: 10.4269/ajtmh.1980.29.941. [DOI] [PubMed] [Google Scholar]
- CASEY H. L. STANDARDIZED DIAGNOSTIC COMPLEMENT FIXATION METHOD AND ADAPTATION TO MICRO TEST. I. LABORATORY BRANCH COMPLEMENT FIXATION METHOD BY LABORATORY BRANCH TASK FORCE. II. ADAPTATION OF LBCF METHOD TO MICRO TECHNIQUE. Public Health Monogr. 1965;74:1–34. [PubMed] [Google Scholar]
- CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
- Casals J., Tignor G. H. The Nairovirus genus: serological relationships. Intervirology. 1980;14(3-4):144–147. doi: 10.1159/000149175. [DOI] [PubMed] [Google Scholar]
- Hoogstraal H. The epidemiology of tick-borne Crimean-Congo hemorrhagic fever in Asia, Europe, and Africa. J Med Entomol. 1979 May 22;15(4):307–417. doi: 10.1093/jmedent/15.4.307. [DOI] [PubMed] [Google Scholar]
- Johnson K. M., Elliott L. H., Heymann D. L. Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys. J Clin Microbiol. 1981 Nov;14(5):527–529. doi: 10.1128/jcm.14.5.527-529.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saluzzo J. F., Aubry P., McCormick J., Digoutte J. P. Haemorrhagic fever caused by Crimean Congo haemorrhagic fever virus in Mauritania. Trans R Soc Trop Med Hyg. 1985;79(2):268–268. doi: 10.1016/0035-9203(85)90356-6. [DOI] [PubMed] [Google Scholar]
- Saluzzo J. F., Digoutte J. P., Camicas J. L., Chauvancy G. Crimean-Congo haemorrhagic fever and Rift Valley fever in south-eastern Mauritania. Lancet. 1985 Jan 12;1(8420):116–116. doi: 10.1016/s0140-6736(85)92014-8. [DOI] [PubMed] [Google Scholar]
- Saluzzo J. F., Digoutte J. P., Cornet M., Baudon D., Roux J., Robert V. Isolation of Crimean-Congo haemorrhagic fever and Rift Valley fever viruses in Upper Volta. Lancet. 1984 May 26;1(8387):1179–1179. doi: 10.1016/s0140-6736(84)91419-3. [DOI] [PubMed] [Google Scholar]
- Suleiman M. N., Muscat-Baron J. M., Harries J. R., Satti A. G., Platt G. S., Bowen E. T., Simpson D. I. Congo/Crimean haemorrhagic fever in Dubai. An outbreak at the Rashid Hospital. Lancet. 1980 Nov 1;2(8201):939–941. [PubMed] [Google Scholar]
- Swanepoel R., Struthers J. K., McGillivray G. M. Reversed passive hemagglutination and inhibition with Rift Valley fever and Crimean-Congo hemorrhagic fever viruses. Am J Trop Med Hyg. 1983 May;32(3):610–617. doi: 10.4269/ajtmh.1983.32.610. [DOI] [PubMed] [Google Scholar]
- van Eeden P. J., van Eeden S. F., Joubert J. R., King J. B., van de Wal B. W., Michell W. L. A nosocomial outbreak of Crimean-Congo haemorrhagic fever at Tygerberg Hospital. Part II. Management of patients. S Afr Med J. 1985 Nov 9;68(10):718–721. [PubMed] [Google Scholar]