Skip to main content
. Author manuscript; available in PMC: 2009 Aug 12.
Published in final edited form as: Nature. 2009 Jan 28;457(7231):892–895. doi: 10.1038/nature07679

Figure 3. Runx1 requirement in blast colony development.

Figure 3

a) Phase contrast time-lapse photographs of Runx1-/- blast colony development. b) FACS analysis of CD41 and c-Kit expression during Runx1-/- blast colony development between day 1 and 4. c) FACS analysis of Tie2 and c-Kit expression during Runx1+/+ (top) and Runx1-/- (bottom) cells blast colony development. d) FACS analysis of Tie2/CD41 expression after 2 days of culture of iRunx1 Runx1-/- Tie2hic-Kit+CD41- cells in absence or presence of doxycycline (dox) (0.1 μg/ml). e) Dil-Ac-LDL and CD41 expression analysis of iRunx1 Runx1-/- Tie2hic-Kit+CD41- cells were evaluated in absence (left) and presence of doxycycline (right). Numbers indicate the percentage of the respective populations. f) Numbers of definitive haematopoietic colonies generated in methylcellulose by iRunx1 Runx1-/- cells harvested after 2 days of culture with or without doxycycline. Error bars indicate standard deviation of the mean (n=3).