Figure 1.

Isolation of a predominantly intramolecularly crosslinked form of fibrinogen, after its reaction with red cell transglutaminase. (Main graph) Gel filtration of transglutaminase-treated fibrinogen. (Inset I) SDS/PAGE profile for the control fibrinogen (φ), obtained under reducing conditions, was compared with that of the material collected in fractions 129–152, marked φ_x. (Inset II) Densitometric scans of the gels shown in I. (Inset III) Nonreducing SDS/agarose electrophoresis of the φ_x pool shows it to be a predominantly monomeric derivative of fibrinogen (φ₁), comprising a small percentage of crosslinked dimers (φ₂).