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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Biochemistry. 2008 Nov 25;47(47):12380–12388. doi: 10.1021/bi801470m

Table 1.

Depth parameters, Φ1, for syt1C2A and syt1C2AB†

Mutant Πoxy ΠNiEDDA Φ1
C2AB L262R1 0.13±0.01 1.40±0.1 −2.4±0.1
C2AB Q263R1 0.14±0.02 1.20±0.1 −2.2±0.1
C2AB S264R1 0.14±0.02 0.99±0.07 −2.2±0.1
C2AB A265R1 0.13±0.02 1.18±0.08 −2.2±0.1
C2AB Q266R1 0.17±0.03 1.17±0.10 −2.2±0.1
C2AB K267R1 0.16±0.02 2.0±0.1 −2.5±0.1
C2AB E268R1 0.22±0.02 2.1±0.1 −2.3±0.1
C2AB E269R1 0.22±0.03 2.2±0.2 −2.3±0.1
†

Depth parameters for the single R1 labeled sites when syt1C2A or syt1C2AB are completely bound to vesicles composed of POPC:POPS (75:25). Errors are based upon fits of the data to P1/2.