Table 2.

Depth parameter, Φ₂, for syt1C2A and syt1C2AB.^†

Mutant	Πoxy	ΠNiEDDA	ΠDOGSNTA	Φ2
C2A A170R1	0.27±0.03	3.0±0.2	0.18±0.02	−2.8±0.1
C2A T176R1	0.24±0.02	2.6±0.2	0.68±0.04	−1.4±0.1
C2A K189R1	0.26±0.02	3.3±0.2	0.07±0.02	−3.9±0.3
C2A F193R1	0.17±0.02	1.4±0.1	0.11±0.02	−2.6±0.2
C2A L202R1	0.30±0.02	4.9±0.3	0.41±0.03	−2.5±0.1
C2A I239R1	0.14±0.01	1.2±0.1	0.89±0.05	−0.3±0.1
C2A I240R1	0.12±0.01	1.6±0.1	0.06±0.01	−3.3±0.2
C2A S217R1	0.40±0.04	3.7±0.2	-	-
C2A T256R1	0.26±0.02	3.4±0.2	0.004±0.01	−6.7±2.2
C2AB L262R1	0.13±0.01	1.4±0.1	0.1±0.01	−2.7±0.1
C2AB Q263R1	0.14±0.02	1.2±0.1	0.1±0.01	−2.6±0.1
C2AB K267R1	0.16±0.02	2.0±0.1	1.10±0.07	−0.6±0.1
C2AB E268R1	0.22±0.02	2.1±0.1	0.57±0.05	−1.3±0.1
C2AB E269R1	0.22±0.03	2.2±0.2	1.39±0.10	−0.5±0.1

^†Depth parameters for the single R1 labeled sites when syt1C2A or syt1C2AB are completely bound to vesicles composed of POPC:POPS or POPC:POPS:DOGS-NTA-Ni(II). Errors are based upon fits of the data to P_1/2.