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. 2009 Apr 6;4(4):e5042. doi: 10.1371/journal.pone.0005042

Figure 4. Inhibition of adhesion to collagen IV by heparinase.

Figure 4

Isogenic strains of Staphylococcus aureus examined for their binding capacity (Y-axis, absorbance reading at 540 nm) to collagen IV (Sigma) coated on 96-well plates, at concentrations of 5 µg/mL. Heparinase III was added at increasing concentrations (X-axis) to collagen IV (Sigma) before the adhesion assay. Parental (LUG960) indicates the genetic background in which the genetic modifications were made; LukS-PV SP, parental carrying a plasmid encoding the LukS-PV signal peptide. The vertical lines indicate the standard deviations.