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. 2009 Apr 6;4(4):e5042. doi: 10.1371/journal.pone.0005042

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Tristan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The 2% SDS extract of various strains were run on 17% SDS PAGE, transferred to nylon membrane and probed with mouse monoclonal anti-poly His antibody (1∶500) (Sigma) followed by goat anti mouse HRP conjugated antibody (1∶2000) (Sigma). Parental (LUG960) indicates the genetic background in which the genetic modifications were made; His-LukS-PV SP, parental carrying a plasmid encoding the His-tagged LukS-PV signal peptide alone; His-LukS-PV, parental carrying a plasmid encoding the His-tagged full length LukS-PV.