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. 2009 Apr 6;4(4):e5042. doi: 10.1371/journal.pone.0005042

Table 2. Plasmids.

Staphylococcal plasmids
pC194 3 kb S. aureus plasmid, inducible chloramphenicol resistance (cat) [46]
pSK265 pC194::pUC19 MCS [36]
pLUG534 pSK265::luk-PV This work
pLUG549 pSK265::Nter LukS-PV (41aa) This work
pLUG560 pSK265::P-luk-PV–TT-luk-PV (PVL vector) This work
pLUG652 pSK265::Cter LukF-PV (128aa) This work
pLUG654 pSK265::LukS-PV signal peptide This work
pLUG670 pSK265::LukS-PVΔ signal peptide This work
pLUG671 pSK265::LukF-PV signal peptide This work
pLUG676 pSK265::TSST-1 signal peptide This work
pLUG725 pSK265::lukF-PV This work
pLUG726 pSK265::lukS-PV This work
pLUG732 pSK265::LukE signal peptide This work
pLUG733 pSK265::LukD signal peptide This work
pLUG737 pSK265::luk-PV mRNA This work
pLUG755 pSK265::mutated LukS-PV signal peptide: R5K This work
pLUG760 pSK265::mutated LukS-PV signal peptide: H24Q This work
pLUG774 pSK265::mutated LukS-PV signal peptide: H24A This work
pLUG775 pSK265::mutated LukS-PV signal peptide: S22P This work
pLUG776 pSK265::shortened LukS-PV signal peptide: [1][25] This work
pLUG777 pSK265::shortened LukS-PV signal peptide: [1][22] This work
pLUG778 pSK265::mutated LukS-PV signal peptide: K27A This work
pLUG786 pSK265::mutated LukS-PV signal peptide: K4N This work
pLUG787 pSK265::mutated LukS-PV signal peptide: K4N/K5A This work
pLUG788 pSK265::mutated LukS-PV signal peptide: K3A/K4N This work
pLUG797 pSK265::chimeric signal peptide: LukS-PV [aa 1–20]::LukE [aa 21–28] This work
pLUG798 pSK265::mutated LukS-PV signal peptide: E25Q This work
pLUG799 pSK265::chimeric signal peptide: LukE [aa 1–20]::LukS-PV [aa 21–28] This work
pLUG886 pORI23::LukS-PV signal peptide This work
pLUG887 pORI23::LukE signal peptide This work
pLUG888 pORI23::LukS-PV This work
pLUG907 pSK265::mutated LukS-PV signal peptide K3A/K4N::LukS-PV This work
pLUG908 pSK265::6xHis::LukS-PV This work
pLUG910 pSK265::chimeric signal peptide: LukE [aa 1–20]::LukS-PV [aa 21–28]::LukS-PV This work
pLUG911 pSK265::mutated LukS-PV signal peptide K4N/K5A::LukS-PV This work
pLUG912 pSK265::6xHis::LukS-PV signal peptide This work
pLUG913 pSK265::chimeric signal peptide: LukS-PV [aa 1–20]::LukE [aa 21–28]::LukS-PV This work