Activation of PAR2 stimulates Na,K-ATPase Vmax in
the rat cTAL. A, Na,K-ATPase Vmax in cTAL
incubated under basal conditions (B, open columns) or with 40
μm PAR2 AP (dark columns) for 5 or 20 min. Values are
means ± S.E. from 4 (20 min) and 13 (5 min) animals. **, p
< 0.001 as compared with basal. B, representative experiment
showing Na,K-ATPase Vmax in cTAL incubated for 5 min at 37
°C in basal condition B or in the presence of 10 nm
Try, 40 μm PAR2 AP, 40 μm inactive RP, or 10
μm F-AP. **, p < 0.001 as compared with basal.
C, Na,K-ATPase activity in cTALs incubated for 5 min at 37 °C
with or without 40 μm AP (B and AP,
respectively) in the absence (Control) or presence of 100
μm furosemide. Values are means ± S.E. from 3
experiments. **, p < 0.001 as compared with basal. D,
representative immunoblot and mean change in plasma membrane expression of
Na,K-ATPase α subunit in cTAL incubated for 10 min at 37 °C under
basal conditions (B) or in the presence of 40 μm PAR2
AP. Membrane Na,K-ATPase was quantified by Western blotting after biotin
labeling and streptavidin precipitation. Values are means ± S.E. from 4
experiments.