TABLE 2.
CCT dimer
|
kcat (n)
|
Km (n)
|
|||
---|---|---|---|---|---|
PCho | CTP | ||||
min-1 | mm | ||||
1 | 367/His236(K122A) | 454 ± 11a (4) | 0.48 ± 0.07 (2) | 1.82 ± 0.16 (2) | |
2 | 367/His367(K122A) | 474 ± 10a (5) | 0.28 ± 0.03 (3) | 0.61 ± 0.08 (2) | |
3 | 367/367 (denatured/renatured) | 861 ± 32 (4) | 0.41 ± 0.10 (2) | 0.72 ± 0.14 (2) | |
4 | 367/367 (untreated) | 885 ± 35 (2) | 0.46 ± 0.12 (2) | 0.64 ± 0.10 (2) |
For data sets with n > 2, t test analyses showed that the probability of being the same as the CCT367/367 dimer on row 3 is <0.005.