FIGURE 2.

Combinatorial expressions of γ3 chain-containing laminins in 293-F cells. 293-F cells were transfected with expression vectors for the γ1 or γ3 chain together with various combinations of expression vectors for α and β chains. At 72 h after transfection, conditioned media (1 or 8 ml) were immunoprecipitated with an anti-FLAG M2 antibody. 8-fold indicates that 8-ml aliquots of conditioned media were immunoprecipitated with the anti-FLAG M2 antibody. Equal amounts of the immunoprecipitates were separated by SDS-PAGE in 4 or 5% gels under reducing conditions, followed by immunoblotting with mAbs against the α1-α5 chains (A) and β1, β2, and γ3 chains (B).