Insecticidal activity of XnGroEL on H. armigera larvae.
A, purified proteins XnGroEL (5–15 μg/g diet) and EcGroEL at
a concentration of 20 μg/g diet were added to the diet of 1-day-old
neonates, and growth and mortality were recorded during 6–12 days of
larval period. B, plot of percent mortality against protein
concentration. Each dot in the plot represents the average of three individual
experiments. The range of protein concentration with high statistical
significance (p < 0.001) was selected for linear regression
analysis; concentration range shown in the plot: XnGroEL (∼16–400
nmol), apical-intermediate (∼30–1300 nmol), apical
(∼40–2000 nmol), and EcGroEL (∼16–700 nmol). The
parameters calculated for each line are as follows: slope (m) 0.006, 0.011,
0.021, and 0.006, intercept (c) 6.638, 7.207, 9.430, and 0.011 for
apical, apical-intermediate, full-length XnGroEL, and EcGroEL proteins,
respectively. WT, wild type.