FIGURE 3.

Insecticidal activity of XnGroEL on H. armigera larvae. A, purified proteins XnGroEL (5–15 μg/g diet) and EcGroEL at a concentration of 20 μg/g diet were added to the diet of 1-day-old neonates, and growth and mortality were recorded during 6–12 days of larval period. B, plot of percent mortality against protein concentration. Each dot in the plot represents the average of three individual experiments. The range of protein concentration with high statistical significance (p < 0.001) was selected for linear regression analysis; concentration range shown in the plot: XnGroEL (∼16–400 nmol), apical-intermediate (∼30–1300 nmol), apical (∼40–2000 nmol), and EcGroEL (∼16–700 nmol). The parameters calculated for each line are as follows: slope (m) 0.006, 0.011, 0.021, and 0.006, intercept (c) 6.638, 7.207, 9.430, and 0.011 for apical, apical-intermediate, full-length XnGroEL, and EcGroEL proteins, respectively. WT, wild type.