Structural analysis of XnGroEL protein. A, comparison of
protein sequences of XnGroEL (AY184491) and EcGroEL (NC_000913) showing the
three domains. Star, equatorial domain; solid squares,
intermediate domain; open squares, apical domain; dash,
undefined. The 59 substitutions are shown by amino acids symbols. The
mutations performed in the study are shown by arrows. XN, X. nematophila.
EC, E. coli. B, phylogenetic tree was constructed by PHYLIP using
ClustalW web server. Wolbachia (AAW52271), Holospora obtusa
(BAA14046), Listeria welshimeri (CAK21507), Sinorhizobium
meliloti (NP_435641), bacterial isolate from Bemisia tabaci
(AAR23105), Pseudomonas aeruginosa (AAB34346), Azotobacter
vinelandii (AAL25964), Buchnera aphidicola (AAO33050), bacterial
isolate from Sitobion avenae (AAB47483), bacterial isolate from
Rhopalosiphum padi (AAB47482), X. nematophila (AY184491),
Haemophilus influenzae (NP_438701), Actinobacillus
actinomycetemcomitans (AAM88539), Ishikawaella (BAF44092),
Wigglesworthia glossinidia (AAK07427), Candidatus
Blochmannia (AAZ40717), bacterial isolate from Sitophilus oryzae
(AB97670), Sodalis glossinidius (AAK92204), Yersinia pestis
(YP_653837), Klebsiella pneumoniae (ABY64753), E. coli
(NC_000913), and E. aerogenes (AAL09389).