Interaction of XnGroEL with larval gut BBMV. The proteins were
incubated with BBMVs, washed, and resolved by SDS-PAGE. Membrane-bound protein
was detected by immunoblotting with antibody (1:20,000) of XnGroEL. A,
lane 1, full-length protein; lane 2, apical domain protein;
lane 3, heated full-length protein; lane 4, EcGroEL;
lane 5, heated apical domain; lane 6, membranes alone;
lane 7, bovine serum albumin. B and C, inhibition
of binding of XnGroEL and apical domain respectively by sugars, 300
mm each. Protein binding was detected as above. NANA,
N-acetylneuraminic acid.