FIGURE 5.

Interaction of XnGroEL with larval gut BBMV. The proteins were incubated with BBMVs, washed, and resolved by SDS-PAGE. Membrane-bound protein was detected by immunoblotting with antibody (1:20,000) of XnGroEL. A, lane 1, full-length protein; lane 2, apical domain protein; lane 3, heated full-length protein; lane 4, EcGroEL; lane 5, heated apical domain; lane 6, membranes alone; lane 7, bovine serum albumin. B and C, inhibition of binding of XnGroEL and apical domain respectively by sugars, 300 mm each. Protein binding was detected as above. NANA, N-acetylneuraminic acid.