FIGURE 5.

Heat-induced lipid peroxidation in the thylakoid and PS II membranes estimated by the TBA test. A, thylakoid membranes. B, PS II membranes. Absorbance at 532 nm of the TBA·MDA complex was measured under the aerobic (closed circles) and anaerobic conditions (open circles) after incubation of the samples at 40 °C for the periods indicated. The concentration of chlorophyll in the reaction mixture was 1 mg ml^-1 chlorophyll in A, and 0.1 mg ml^-1 chlorophyll in B, respectively. The data are the average of three independent measurements ± S.D.