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Temperature and power dependence of EPR spectra of reconstituted holoNfuA. The EPR spectrum of holo-NfuA at 16 K and 40 milliwatts shows the axial line shape of an 4Fe-4S cluster with a low-field peak at g = 2.037 and a high-field trough at g = 1.903. Inset, temperature dependence (in Kelvins) of the holo-NfuA EPR signal at different microwave powers: 126 milliwatts (circles), 40 milliwatts (squares), 10 milliwatts (triangles), 1 milliwatt (diamonds), and 0.1 milliwatt (bars).
